Cytiva Cell Proliferation Kit, BioTrak™ ELISA Kit and Components

Product Code. 10004694

Brand: Cytiva RPN202

Description

Includes

The cell proliferation kit includes:
Labeling reagent, Nuclease, Anti-5-bromo-2'-deoxyuridine monoclonal antibody, Peroxidase anti-mouse IgG2a, Peroxidase anti-mouse IgG2a diluent, Substrate/intensifier

Cel proliferation Biotrak ELISA kit includes:
BrdU labelling reagent; Fixative; Blocking reagent; Peroxidase-labelled anti-BrdU; Antibody dilution solution; Wash buffer; Substrate solution

Cell proliferation labelling reagent and Anti-BrdU antibody are available separately

Provides a precise, fast and simple colorimetric assay to quantitate proliferating cells cultured in a 96 well microplate

• Recommended cell number: 10e6-10e7 cells/mL (Cell proliferation kit) 10e2 cells/well-10e5 cells/well (Biotrak ELISA kit)
• Required time: 2-3 hour post-labeling (labeling 1-2 hours)
• Antibody and labeling reagent available separately for ELISA and ICC systems
• Suitable for in vivo and in vitro tissue labeling in addition to cultured cells (ELISA format)
• Non radioactive protocols provide sensitivity comparable to autoradiographic methods
• Cell proliferation kit avoids the need to chemically denature DNA. Cellular morphology is preserved allowing multiple labeling experiments
• 5-fluoro-2'-deoxyuridine is used to inhibit thymidilate synthetase increasing BrdU incorporation

When ordering the Cell Proliferation Kit (RPN20), you must also order DAB REAGENT OF RPN20/RPN1174 (RPNK8888)

Spezifikation

• Antigen: BrdU
• Includes: Anti-5-2'-deoxyuridine Monoclonal Antibody Nuclease
• Quantity: 130 μL
• Content And Storage: 2°C to 8°C
• For Use With (Application): Immunocytochemistry

Sicherheit und Handhabung

Signalwort

• Danger

Gefahrenkategorie

• Respiratory sensitiser Category 1
• Skin sensitiser Category 1
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1

Gefahrenhinweis

• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Sicherheitshinweis

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P310-Immediately call a POISON CENTER/doctor/.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Ergänzende Informationen

• MIXTURE LIST-Contains : deoxyribotrometamolnuclease