Invitrogen™ Bovine IL-4 Uncoated ELISA Kit

Product Code.	Quantity	unitSize
10177433	5 x 96 Tests	1 set

Product Code. 10177433 Supplier Invitrogen™ Supplier No. ESS0031

Description

ELISA

The Bovine IL-4 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-4. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P30367
• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: <15.6 pg/mL
• Description: Bovine IL-4 ELISA Reagent Kit
• Conjugate: HRP
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Specificity: No significant cross-reaction with recombinant bovine IL-1ß, IL-2, IL-6, IL-8, IFNg or TNFa; no significant cross-reaction with recombinant human, equine, mouse, porcine, rat, or rhesus macaque IL-4
• Sufficient For: 5 x 96 microplate wells
• Target Species: Bovine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 280824
• Gene Symbol: BSF-1,IL-4
• Kit Contents: Coating Antibody, Lyophilized Recombinant Standard, Detection Antibody, Streptavidin-HRP, Substrate Solution, Ready to use Stop Solution, 0.16M Sulfuric Acid, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
• Label or Dye: HRP
• Measurement: Absorbance at 450 nm minus absorbance (at 550 nm)
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-4
• Test Time: 24 hrs
• Total Assay Time: 3 hrs 20 mins