Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)

Go from Biological Sample to Genotyping Results in less than one hour.

Brand:  Applied Biosystems™ 4403083

Product Code. 10045264

Includes: Reagents sufficient for 4,000 sample preparation reactions at 50μL/ reaction and 4,000 PCR reactions at 5μL for each reaction.

Description

The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
• The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

• Content And Storage: Contains Lysis Reagents (2° to 8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C): 10 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• GC-Rich PCR Performance: Low
• Polymerase: AmpliTaq DNA Polymerase
• Technique: SNP Genotyping (Real-Time PCR-Based)
• Genotyping Target: SNPs (Unknown or Numerous), SNPs (Known)
• Product Line: Sample-to-SNP™, TaqMan™
• Quantity: 1 kit
• Hot Start: Built-In Hot Start
• Volume: 20 mL

• Detection Method: Primer-probe
• PCR Method: qPCR
• Reaction Speed: Fast or Standard
• For Use With (Equipment): 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Fast Mode, StepOne™ Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Genotyping Reagents
• Sufficient For: 400 Reactions
• Sample Type: Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood

For Research Use Only. Not for use in diagnostic procedures.