Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Product Code.	Quantity	unitSize
10043102	60 Assays	5mL

Product Code. 10043102

Brand: Molecular Probes™ A23210

Description

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

Specifications

• Color: Green
• Content And Storage: Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).
  
  Store as directed.
• Detection Method: Fluorescence
• Format: Tube
• Excitation/Emission: 490/525
• Flow Cytometer Laser Lines: 488
• For Use With (Application): Flow Cytometry
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Label or Dye: Alexa Fluor™ 488
• Product Type: TUNEL Assay Kit
• Product Line: APO-BrdU
• Quantity: 60 Assays
• Species: Mouse
• Target: BrdU
• Label Type: Alexa Fluor Dyes

Safety and Handling

Product Identifier

• APO-BrdU TUNEL Assay Kit, with Alexa Fluor 488 Anti-BrdU

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P240-Ground/bond container and receiving equipment.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P242-Use only non-sparking tools.
• P243-Take precautionary measures against static discharge.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.