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Invitrogen™ V5 Tag Monoclonal Antibody, AP

Product Code. 10103862
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125 μL
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10103862 125 μL Each
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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 
Product Code. 10103862 Supplier Invitrogen™ Supplier No. R96225

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Mouse Monoclonal Antibody

NOTE: R962-25 was previously sold under product #46-0287. When re-ordering, please use #R962-25. R962-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk. In Western blot applications, no cross-reactivity has been observed in bacterial lysates.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
TRUSTED_SUSTAINABILITY

Specifications

Antigen V5 Tag
Applications Western Blot
Classification Monoclonal
Concentration 1-2 mg/mL
Conjugate Alkaline Phosphatase (AP)
Formulation PBS with 0.01% sodium azide
Gene Alias simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag
Host Species Mouse
Immunogen V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.
Purification Method Purified
Quantity 125 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG2a
Target Molecule Tag
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