Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit

Product Code. 10196864

Brand: Invitrogen™ AM1914

Description

Includes

600μL 10X DNase I Buffer; 45μL DNase I 2U/μL; 1mL Formaldehyde Load Dye; 100μL Linear Acrylamide 5mg/mL; 50μL Positive Control Primers; 1mL 5M Ammonium Acetate; 35mL Water for 64% Ethanol; 600mL DNase Inactivation Reagent; 30mL Lysis/Binding Solution; 25mL Wash Solution 1; 35mL Wash Solution 2/3; 3 Pestles for tissue disruption; 30 Filter Cartridges; 60 Collection Tubes; 25mL Elution Solution

The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 10⁷ cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 10⁷ cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.

• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations

Using the RNAqueous-4PCR Kit

The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications

• Content And Storage: • 600 μL 10X DNase 1 Buffer; store at -20°C
  • 45 μL DNase 1; -20°C
  • 1 mL Formaldehye Load Dye; -20°C
  • 100 μL Linear Acrylamide; -20°C
  • 50 μL Positive Control Primers; -20°C
  • 1 mL 5M Ammonium Acetate; -20°C
  • 35 mL Water; 4°C
  • 600 μL DNase Inactivation Reagent; 4°C
  • 30 mL Lysis/Binding Solution; 4°C
  • 25 mL Wash Solution #1; 4°C
  • 35 mL Wash Solution 2/3 (concentrated); 4°C
  • 3 Pestles; room temperature
  • 30 Filter Cartridges; room temperature
  • 60 Collection Tubes; room temperature
  • 25 mL Elution Solution; room temperature
• Isolation Technology: Silica Spin Column
• Purification Time: 10 min. (After sample homogenization)
• Elution Volume: 50–120 μL
• Sample Type: Tissue, Cells
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 30 Preps
• Shipping Condition: Box 1: Dry Ice, Box 2: Room Temperature
• Starting Material Amount: Tissue: ≤75 mg, Cells: ≤10⁷
• Yield: ≤10 μg per 1 mg tissue, ≤1 μg per 1 x 10⁵ cells

Safety and Handling

Product Identifier

• RNAqueous-4PCR Total RNA Isolation Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 1B
• Serious eye damage/eye irritation Category 2
• Germ cell mutagenicity Category 1B
• Germ cell mutagenicity Category 2
• Reproductive toxicity Category 1B
• Reproductive toxicity Category 2
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H340-May cause genetic defects.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H360-May damage fertility or the unborn child.
• H361f-Suspected of damaging fertility.
• H371-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 2-Mercaptoethanol, Formamide, Formaldehyde, Methanol, Acrylamide

For Research Use Only. Not for use in diagnostic procedures.