Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Pricing and Availability
Specifications

Cut Site	CTCTTC(1/4)^
Content And Storage	Store at -20°C.
Form	Liquid
Enzyme	Eam1104I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity	3000 U, 150 μL
Type IIS RE	Yes
Incubator Temperature	37°C
Concentration	20 U/μL
For Use With (Application)	In vitro Transcription
Inactivation Temperature	65°C
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Research Category	Drug Development
Compatible Buffer	10X Tango Buffer
Optimal Reaction Temperature	37°C
Product Line	TheraPure™

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

Pricing and Availability

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

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Thermo Scientific™ AarI (2 U/μL)

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	AarI
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Unique Buffer (10X Buffer AarI)
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	2 U/μL

Thermo Scientific™ BglI (10 U/μL)

Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Bgl I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer O
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ NheI (10 U/μL)

The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ HinfI (10 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Hinf I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Thermo Scientific™ SmaI (10 U/μL)

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).

Pricing and Availability
Specifications

Concentration	10 U/μL

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Thermo Scientific™ FastDigest ScaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Pricing and Availability

Thermo Scientific™ FastDigest Bsp119I

Cut at TT^CGAA sites with Thermo Scientific™ FastDigest™ Bsp119I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Thermo Scientific™ FastDigest XmaJI

Cut at C^CTAGG sites with Thermo Scientific™ FastDigest™ AvrII (XmaJI), which performs best using universal FastDigest Buffer (Isoschizomers: AspA2I, BlnI).

Pricing and Availability

Thermo Scientific™ PfeI (TfiI) (10 U/μL)

Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).

Pricing and Availability

Thermo Scientific™ FspBI (BfaI) (10 U/μL)

The FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BfaI, MaeI, XspI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	FspBI (BfaI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

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Thermo Scientific™ TaqI (10 U/μL)

The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.

Pricing and Availability
Specifications

Concentration	10 U/μL

Thermo Scientific™ FastDigest AatII

Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).

Pricing and Availability

Restriction Enzymes

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