Protein Mass Spectrometry Sample Prep
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Thermo Scientific™ High Select™ Phosphopeptide Enrichment Kits & Reagents
The expanded High Select family of complete kits supports enrichment of phosphorylated peptides using Fe-NTA and TiO2 immobilized resins in spin column, tip, and magnetic bead formats designed for use with MS analysis.
Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit
Increase protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit.
Thermo Scientific™ Pierce™ Quantitative Peptide Assays & Standards
Pierce colorimetric or fluorescent quantitative peptide assays and standards are easy-to-use microplate assays designed specifically to improve the sensitivity and reproducibility of peptide quantitation for use with mass spectrometry analysis.
| Workflow Step | In-Process Testing |
|---|---|
| For Use With (Equipment) | Microplate Reader |
| Starting Material | Peptides,Protease-digested Protein |
| Product Line | Pierce |
Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay
Measure total peptide concentration by a mix-and-read fluorescent microplate method that provides improved sensitivity, reproducibility, and ease using the Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay.
Thermo Scientific™ EasyPep™ MS Sample Prep Kits
Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
| Starting Material | Protein Samples |
|---|---|
| Detection Method | Mass Spectrometry |
| Product Line | EasyPep |
Thermo Scientific™ Tryptic Digestion Kits
Improve peptide digest quality and sensitivity of MS detection using thesw Thermo Scientific Digestion Kits for efficient and reproducible preparation of peptide digests.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Detection Method | Mass Spectrometry |
Thermo Scientific™ Pierce™ Albumin Serum Depletion Kits
Optimize serum sample prep by depleting unwanted albumin and IgG serum proteins for protein-profiling methods using quality depletion kits.
| Final Product Type | Protein |
|---|---|
| Starting Material | Plasma,Serum |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kits
Achieve highly efficient, compatible immunoprecipitation and co-immunoprecipitation of target antigens for downstream LC-MS analysis using Thermo Scientific Pierce MS-Compatible Magnetic IP Kits with streptavidin or Protein A/G magnetic beads.
| Format | Kit |
|---|---|
| Workflow Step | Sample Enrichment |
| Final Product Type | Protein |
| Product Type | Magnetic IP Kit |
| For Use With (Equipment) | KingFisher™ Flex, Magnetic Stand |
| Sufficient For | 40 Reactions |
| Quantity | 40 reactions kit |
| For Use With (Application) | Mass Spectrometry |
| Starting Material | Protein Samples,Cell Lysate |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ SureQuant™ IP-MS Sample Preparation Kits
Thermo Scientific SureQuant IP-MS Sample Preparation Kits provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis.
| Content And Storage | Store trypsin at 4°C. Store trypsin storage solution at -20°C. |
|---|---|
| Workflow Step | Sample prep enrichment |
| For Use With (Equipment) | Mass spectrometer |
| Quantity | 20 Reactions |
| For Use With (Application) | MS-compatible immunoprecipitation |
| Starting Material | Cells,Tissue |
| Detection Method | Mass Spectrometry |
| Product Line | SureQuant |
Thermo Scientific™ High-Select™ Fe-NTA Magnetic Phosphopeptide Enrichment Kit
The Thermo Scientific™ High-Select™ Fe-NTA Magnetic Phosphopeptide Enrichment Kits use an advanced iron-chelate magnetic bead system designed for rapid and highly specific enrichment of phosphorylated peptides from complex biological samples.
| Shipping Condition | Wet Ice |
|---|---|
| Format | Kit |
| Workflow Step | Peptide Enrichment |
| Form | Solid |
| Final Product Type | Peptides |
| Product Type | Fe-NTA Phosphopeptide Enrichment |
| Starting Material | Protease-digested Protein |
| Detection Method | Mass Spectrometry |
| Product Line | High Select |
Thermo Scientific™ EasyPep™ Magnetic MS Sample Preparation Kits and Proteomics Magnetic Clean-up Beads
EasyPep Magnetic MS Sample Preparation Kits and Proteomics Magnetic Clean-up Beads contain MS-compatible magnetic beads specifically designed to tolerate harsh MS sample prep conditions to improve sample quality and reproducibility for both manual and automated sample preparation methods.
| Form | Liquid |
|---|---|
| Starting Material | Protein Samples |
| Detection Method | Mass Spectrometry |
Thermo Scientific™ Pierce™ Protein Precipitation Plates
Pierce Protein Precipitation Plates are used for protein processing via simple, rapid and automatable protein precipitation and removal of proteins from plasma, serum and other biological fluid samples alongside the Rapid Equilibrium Device (RED) for plasma protein-binding assays.
| Content And Storage | Upon receipt store plates at room temperature. Product shipped at ambient temperature. |
|---|---|
| Format | Plate |
| Workflow Step | Sample Clean Up |
| Final Product Type | Peptides |
| Product Type | Protein Precipitation Plate |
| For Use With (Equipment) | Mass Spectrometer, Centrifuge |
| Starting Material | Protease-digested Protein |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ High Select™ Depletion Spin Columns
Decrease albumin, immunoglobulins, and other high-abundance proteins from human serum or plasma in preparation for mass spectrometry analysis, or 1D and 2D gel electrophoresis using High Select depletion spin columns or resin.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store at 2–8°C. |
| Workflow Step | Protein Depletion |
| Final Product Type | Protein |
| Label or Dye | Unlabeled |
| Starting Material | Plasma,Serum |
| Product Line | High Select |
Thermo Scientific™ Pierce™ Fe-NTA Phosphopeptide Enrichment Kit
Purify phosphorylated peptides to process protein digests or cation-exchange fractions for mass spectrometry using spin columns of iron-chelate agarose resin.