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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.

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2

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Substrate CSPD™
Substrate Properties Chemical Substrate
Content And Storage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format 96-well Plate
For Use With (Equipment) Luminometer (Microplate)
Product Type Phosphatase Assay
Target Enzyme Placental Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility High-throughput Compatible
Product Line NovaBright
Detection Method Enhanced Chemiluminescence
3
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Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System

Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.

EDGE
Content And Storage This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase.
Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate CSPD™
Assay Reporter Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line NovaBright, Phospha-Light
6

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System

Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Luciferase
Substrate Type Luciferase Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Luciferin
Assay Luciferase Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type Luciferase Reporter Gene Assay System
Sample Type Cell Cultures
Product Line Luc-Screen, NovaBright
7

Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.

Content And Storage This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Plus™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
8

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Gal-Screen, NovaBright
9
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Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates. SensitiveThe chemiluminescent assay exhibits over three o

Content And Storage This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer (Microplate)
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Galacto-Star, NovaBright
10

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

11
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Invitrogen™ MessageAmp™ II aRNA Amplification Kit

Amplifies as little as 100pg of input RNA for GeneChip™ analysis

Format Kit
Reverse Transcriptase ArrayScript™
Includes Label or Dye No
Product Type aRNA Amplification Kit
Sample Type Poly(A+) RNA, Total RNA
Product Line Ambion, MessageAmp
12
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Invitrogen™ GLOBINclear™-Human Kit, for globin mRNA depletion

A rapid and robust treatment for RNA derived from whole blood that uses a novel, non-enzymatic technology to rapidly deplete >95% of the alpha and beta globin mRNA from total RNA preparations.

13
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Invitrogen™ FluoReporter™ lacZ Flow Cytometry Kit

FluoReporter™ lacZ Flow Cytometry Kit

14

Invitrogen™ Amino Allyl MessageAmp™ II aRNA Amplification Kit

Provides reagents for aRNA amplification with Amino allyl NTP incorporation

15
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Invitrogen™ FluoReporter™ lacZ/Galactosidase Quantitation Kit

Reaction produces blue fluorescent product