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Zap-70, PE, clone: 1E7.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-6695-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15547076

  • £149.61 / 100 tests


Estimated Shipment date
from Supplier 28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCRζ-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP-70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 282-307. While ZAP-70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP-70 positive expression with mutated IgVH expression in B-chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP-70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B-CLL.Applications Reported: The 1E7.2 antibody has been reported for use in intracellular flow cytometric analysis.Applications Tested: This 1E7.2 antibody is offered in 2 formats: - μg size: has been tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

ZAP-70
1E7.2
PE
Conjugated
IgG1
RUO
Primary
Human
Flow Cytometry (Intracellular Staining)
0.2mg/mL; 5μL (0.5μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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