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ZAP-70 Mouse anti-Human, PE, Clone: 1E7.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-6695-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15547076

  • £146.13 / 100 tests


Estimated Shipment date
from Supplier 26-10-2017
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Disclaimers:

For Research Use Only.


Description

Description

The 1E7.2 antibody reacts with human and mouse ZAP-70, the TCRζ-associated protein-70. ZAP-70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP-70 interacts with the TCR complex by binding to tyrosine-phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP-70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1-3. Tyrosine phosphorylation of ZAP-70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP-70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 282-307. While ZAP-70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP-70 positive expression with mutated IgVH expression in B-chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP-70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B-CLL.
Specifications

Specifications

ZAP-70
1E7.2
PE
Conjugated
IgG1
RUO
Primary
Human
Flow Cytometry (Intracellular Staining)
0.2mg/mL; 5μL (0.5μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal