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Vinculin Monoclonal Antibody (7F9), eFluor™ 570, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£149.00 - £406.00

Specifications

Clone 7F9
Concentration 0.2 mg/mL
Classification Monoclonal
Conjugate eFluor 570
Form Liquid
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Product Code Brand Quantity Price Quantity & Availability  
15558976
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Affymetrix eBioscience
41-9777-82
100 μg
£406.00
100µg
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15548976
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Affymetrix eBioscience
41-9777-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis. Applications Reported: This 7F9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This 7F9 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Vinculin Monoclonal antibody specifically detects Vinculin in Human, Mouse, Rat samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).
Specifications

Specifications

7F9
Monoclonal
Liquid
RUO
P18206, P85972, Q64727
VCL
Primary
4° C, store in dark, DO NOT FREEZE!
VCL
0.2 mg/mL
eFluor 570
Mouse
Human, Mouse, Rat
22330, 305679, 7414
IgG1 κ
Affinity chromatography
Antibody
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For Research Use Only.