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Vinculin, eFluor 570, clone: 7F9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-9777-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15558976

  • £170.84 / 100µg


EU Stock 3
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Description

Description

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.Applications Reported: This 7F9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This 7F9 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

vinculin
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q64727, P85972, P18206
RP11-178G16.3, CMD1W, MVCL
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Microscopy
7F9
eFluor 570
VCL
Conjugated
Vcl
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
7414
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