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Vinculin, Biotin, clone: 7F9, eBioscience™

Mouse Monoclonal Antibody

£69.00 - £165.00

Specifications

Antigen vinculin
Clone 7F9
Target Species Human, Murine, Rat
Conjugate Biotin
Isotype IgG1, kappa, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15567386
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Affymetrix eBioscience
13-9777-80
25μg
£69.00
25µg
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Estimated Shipment
04-03-2021
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15577386
View Documents
Affymetrix eBioscience
13-9777-82
100μg
£165.00
100µg
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Save Up to 
»
Estimated Shipment
04-03-2021
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Description

Description

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.Applications Reported: This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

vinculin
Human, Murine, Rat
IgG1, kappa, kappa
Monoclonal
Liquid
Antibody
Store at 2-8°C. Do not freeze.
7F9
Biotin
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.