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Vinculin Monoclonal Antibody (7F9), Biotin, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-9777-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis. Applications Reported: This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.
Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis.Applications Reported: This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.Specifications
vinculin | |
Monoclonal | |
0.5mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P18206, P85972, Q64727 | |
VCL | |
Affinity chromatography | |
RUO | |
22330, 305679, 7414 | |
Store at 2-8°C. Do not freeze. | |
Liquid |
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) | |
7F9 | |
Biotin | |
VCL | |
RP11-178G16.3, CMD1W, MVCL | |
Mouse | |
25 μg | |
Primary | |
Human, Mouse, Rat | |
Antibody | |
IgG1 κ |
For Research Use Only.