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Thermo Scientific™ Verso One-Step RT-qPCR Kit, SYBR Green, With Separate ROX Vial

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Brand:  Thermo Scientific™ AB4104C

UNSPSC : 41106615

Code : LE

Additional Details : Weight : 0.06000kg

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Product Code. 11822143

  • £830.00 / 400 reactions


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

  • Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
  • RT and qPCR steps performed in single tube - convenience and reduced contamination risk
  • Use of the entire RT reaction as template for qPCR - improved sensitivity
  • Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg
  • Verso RT enzyme has a wide working temperature range (42° to 57°C) - improved success with GC-rich and other difficult templates
  • Contains an inert blue dye for ease of pipetting during reaction setup
Specifications

Specifications

With separate ROX vial
Real time PCR (qPCR), gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, disease research
1 x 50μL Verso Enzyme Mix; 1 x 250μL RT enhancer; 2 x 1.25mL 2X 1-Step qPCR SYBR Mix; 1 x 25μL ROX, 1mM; 1 x 100μL 1M MgCl2. Sufficient for 200 x 25μL reactions.
400 x 25┬ÁL Rxns
Store at -20°C.
SYBR
Bio-Rad CFX384 Touch™, BioRad CFX96™, Cepheid SmartCycler, Corbett Rotor-Gene, Eppendorf MasterCycler RealPlex, MJ Chromo4, MJ Opticon, Roche LightCycler 480
1-Step QRT-PCR Kit w/SYBR Green plus separate ROX vial
RNA
400 Reactions
Documentation

Technical Documents