Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits

Perform 1-step RT-qPCR using probe chemistry with these ready-to-use, optimized solutions.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: AB-4100/A

200RXN VERSO 1-STEP QRT-PCR KIT PLUABsolute, Real-time Reverse transcriptase-PCR, 2

UNSPSC: 41106300

Code: LE

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Code. 11832133

Quantity Price
1 £ 403.0 / 200 reactions
EU Stock 25
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Description and Specification

Specification

Includes Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase1, High performance dNTPS, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)
Product Type 1-Step QRT-PCR Kit plus separate ROX vial
Quantity 200 x 25µL Rxns
Storage Requirements Store at -20°C. Avoid freeze/thaw cycles

Verso 1-Step Kits are designed for minimal hands-on time and faster results. The RT and qPCR reaction components are together in a single tube, reducing protocol times and minimizing the number of pipetting steps. The buffer system of the Verso 1-Step qRT-PCR Kits has been specifically optimized to work across a wide range of RNA templates for both transcription and amplification. Special enhancers and additives have been used to generate a reaction mixture which allows both reverse transcriptase and DNA polymerase to work optimally.
  • Optimized buffer—Verso RT-qPCR Kits are based on a balanced buffer to maximize performance of both the reverse transcriptase and DNA polymerase enzymes

  • Easy-to-see mix—The Verso RT-qPCR Kit contains an inert blue dye for easy sample visualization during reaction set-up

  • Superior dynamic range—Kit enables the detection of 10pg to 1μg of total RNA or 1pg to 10ng mRNA, allowing for accurate detection and quantification of target RNA over a broad template range and ensuring that increases in target RNA concentration give rise to corresponding increases in qPCR signal

  • Maximum specificity—The chemically modified Thermo-Start™ Taq DNA Polymerase remains completely inactive during the reverse transcription stage; the enzyme is then fully activated at the start of the qPCR reaction by a 95°C heating step

  • Broad template range—Verso 1-Step RT-qPCR Kit can detect 10pg-1μg of total RNA or 1pg-10ng of mRNA

  • Easily adapted for automation—The simple 1-step protocol is compatible with robotic systems and reduces hands-on time

  • Unique RT Enhancer—Verso RT-qPCR Kits contain RT Enhancer, a unique enzyme that degrades any contaminating genomic DNA during the RT step and eliminates the need for a DNase I treatment of the extracted RNA, saving time and effort

  • Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

  • Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

Includes:

Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase1, High performance dNTPS, ROX™ passive reference dye (only in ROX mixes); Separate ROX vial (only in kits indicated)

Compatible with:

These blue mixes are ideal for use with white reaction plates and tubes. An increase in detection efficiency is seen when using ABgene white PCR plastics for qPCR. While white plastics give superior qPCR results, they can make traditional colorless reagents difficult to see. With Verso, a simple glance ensures that the correct amount of master mix has been added.

Check qPCR cycler compatibility with our qPCR reagent selection guide.

Recommended for:

A range of applications including gene expression analysis, siRNA knockdown validation, miRNA detection, microarray validation, pathogen detection, and disease research

Documentation

Technical documents