VEGF Receptor 3, clone: AFL4, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5988-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified AFL4, cat. 16-5988, in functional assays.).Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|VEGF Receptor 3|
|PBS with 0.09% sodium azide; pH 7.2|
|FLT41, PCL, FLT4|
|Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.