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VEGF Receptor 3, Biotin, clone: AFL4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5988-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15209989

  • £55.80 / 50µg


Estimated Shipment date
from Supplier 27-06-2018
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Description

Description

Description: The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.Applications Reported: The AFL4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

VEGF Receptor 3
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P35917
FLT41, PCL, FLT4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
AFL4
Biotin
Flt4
Conjugated
Flt4
IgG2a, kappa
50μg
Store at 2-8°C. Do not freeze.
Primary
14257
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