TCR V gamma 2, PerCP-eFluor™ 710, clone: UC3-10A6, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5828-80

Product Code. 15589276

Description

Description: The UC3-10A6 monoclonal antibody reacts with the mouse V gamma 2 (Vg2) delta chain of the gamma delta T cell receptor (TCR). The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood. Studies suggest that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulating the immune response during bacterial infection.Applications Reported: This UC3-10A6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UC3-10A6 antibody has been tested by flow cytometric analysis of C57/Bl splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: V gamma 2 TCR
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Alias: TCRV gamma 2, TCRV y2, TCRV gamma2, TCR V gamma2, Vg2, Vgamma2
• Isotype: IgG
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: UC3-10A6
• Conjugate: PerCP-eFluor 710
• Format: Conjugated
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.