TCR V beta 5.1, PE-Cyanine7, clone: LC4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 25-5832-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This LC4 monoclonal antibody recognizes the human T cell receptor (TCR) Vbeta5.1 allele. Composed of an alpha and beta chain, TCR specificity is typically determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement. Vbeta expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection. More recently, assessment of TCR Vbeta expression has been used to phenotype T cell clonality in CD3+/TCRalpha beta+ large granular lymphocyte leukemias. A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells.This monoclonal antibody has been reported to induce apoptosis and calcium flux.Applications Reported: This LC4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LC4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.25μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|V beta 5.1 TCR|
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|TCRV beta 5.1, TCRV beta5.1, TCR V beta5.1, Vb5.1, Vbeta5.1|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.