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TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£99.75 - £295.00

Specifications

Antigen TCR V beta 5.1/5.2
Clone MR9-4
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
15301360
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Affymetrix eBioscience
46-5796-82
100 μg
£295.00
100µg
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15391350
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Affymetrix eBioscience
46-5796-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports also indicate that V beta 5+ T cells recognize the OVA peptide. Applications Reported: This MR9-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MR9-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

TCR V beta 5.1/5.2 Monoclonal antibody specifically detects TCR V beta 5.1/5.2 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

TCR V beta 5.1/5.2
0.2 mg/mL
Monoclonal
Liquid
RUO
IgG1 κ
Affinity chromatography
Antibody
MR9-4
Flow Cytometry
PerCP-eFluor 710
Mouse
Mouse
Primary
4° C, store in dark, DO NOT FREEZE!
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