TCR V beta 5.1/5.2, eFluor 450, clone: MR9-4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-5796-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports also indicate that V beta 5+ T cells recognize the OVA peptide.Applications Reported: This MR9-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MR9-4 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|V beta 5.1/5.2 TCR|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TCRV beta 5.1, TCRV beta 5.2, TCRV beta5.1, TCRV beta5.2, TCR V beta5.1, TCR V beta5.2, Vb5.1, Vbeta5.1, Vb5.2, Vbeta5.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.