TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15598126	Affymetrix eBioscience 17-5796-80	25 μg
15508136	Affymetrix eBioscience 17-5796-82	100 μg

Description

Description: This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports also indicate that V beta 5+ T cells recognize the OVA peptide. Applications Reported: This MR9-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MR9-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

TCR V beta 5.1/5.2 Monoclonal antibody specifically detects TCR V beta 5.1/5.2 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: TCR V beta 5.1/5.2
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: MR9-4
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Mouse
• Gene Alias: TCR V beta5.1; TCR V beta5.2; TCRV beta 5.1; TCRV beta 5.2; TCRV beta5.1; TCRV beta5.2; Vb5.1; Vb5.2; Vbeta5.1; Vbeta5.2
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.