TCR V beta 11, APC, clone: RR3-15, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 17-5827-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity.The RR3-15 antibody has been reported to activate V beta 11+ T cells.Applications Reported: This RR3-15 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RR3-15 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|V beta 11 TCR|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TCRV beta 11, TCRV beta11, TCR V beta11, Vb11, Vbeta11|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.