TCR V beta 10b, PE, clone: B21.5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5805-81

Product Code. 15248839

Description

Description: The B21.5 monoclonal antibody reacts with the Vbeta10 T Cell Receptor (TCR) in mice of the b haplotype of the TCR Vbeta gene complex, which includes AKR, BALB/c, CBA, C3H/He, C57BL, DBA/1 and DBA/2 mice. It does not react with Vbeta10 in mice of the a or c haplotypes of the TCR Vbeta gene complex, including C57BR, C57L, SJL, and SWR mice.Applications Reported: This B21.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B21.5 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: V beta 10b TCR
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Format: Conjugated
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: B21.5
• Conjugate: PE
• Gene: Tcrb
• Gene Symbols: Tcrb
• Isotype: IgG2a, lambda
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 21577

For Research Use Only.