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TCR V alpha 3.2, PerCP-eFluor™ 710, clone: RR3-16, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5799-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341360

  • £195.75 / 100µg


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Description

Description

Description: This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, and NOD) in the Tcra gene complex. The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1 and DBA/2) haplotypes. Studies demonstrate that the V alpha 3.2 TCR is more highly expressed on CD8+ T cells.Applications Reported: This RR3-16 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RR3-16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TCR V alpha 3.2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
TCRV alpha 3.2, TCRV alpha3.2, TCR V alpha3.2, Valpha3.2, Va3.2
IgG2b, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Murine
Flow Cytometry
RR3-16
PerCP-eFluor 710
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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