TCR V alpha 3.2, FITC, clone: RR3-16, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-5799-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, and NOD) in the Tcra gene complex. The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1 and DBA/2) haplotypes. Studies demonstrate that the V alpha 3.2 TCR is more highly expressed on CD8+ T cells.Applications Reported: This RR3-16 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RR3-16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|V alpha 3.2 TCR|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TCRV alpha 3.2, TCRV alpha3.2, TCR V alpha3.2, Valpha3.2, Va3.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.