TCR V alpha 11, APC, clone: RR8-1, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 17-5800-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This RR8-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR8-1 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b (e.g., C57BL/6, B10) and d (e.g., DBA/1, DBA/2) haplotypes. V alpha 11+ T cells are absent in strains with the a (e.g., BALB/c, C3H) or c (e.g., SJL) haplotypes of the Tcra gene complex. Finally, V alpha 11 is expressed predominantly on CD4+ T cells.The RR8-1 antibody recognizes the V alpha 11.1 and 11.2 alleles, but not 11.3. This antibody has been reported to stimulate V alpha 11+ T cells.Applications Reported: This RR8-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RR8-1 antibody has been tested by flow cytometric analysis of mouse lymph node. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|V alpha 11 TCR|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|TCRV alpha 11, TCRV alpha11, TCR V alpha11, Valpha11, Va11|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.