Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-6078-80

Product Code. 15299999

Description

Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin. Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoblotting (WB). Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunoblotting of treated cell lines. This can be used at less than or equal to 0.5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.

Specifications

• Antigen: Ubiquitin
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P62979, P62982, P62983
• Gene Symbols: RPS27A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100912032, 6233, 78294
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Western Blot
• Clone: eBioP4D1 (P4D1)
• Conjugate: Biotin
• Gene: RPS27A
• Gene Alias: 60S ribosomal protein L40, HUBCEP52, RPL40, Ubiquitin 52 amino acid fusion protein, Ubiquitin A52 residue ribosomal protein fusion product 1, Ubiquiti
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: All species
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.