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TRA-1-60 (Podocalyxin) Mouse anti-Human, PerCP-eFluor™ 710, Clone: TRA-1-60, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-8863-82

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15599376

  • £240.00 / 100µg

Estimated Shipment date
from Supplier 10-03-2020

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Description

Description

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications

Specifications

TRA-1-60 (podocalyxin)
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-60; TRA160
IgM
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Human
Flow Cytometry
TRA-1-60
PerCP-eFluor™ 710
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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