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TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£76.75 - £195.00

Specifications

Clone TRA-1-60
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Classification Monoclonal
Conjugate Unconjugated
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15258107
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Affymetrix eBioscience
14-8863-82
100 μg
£195.00
100µg
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15248107
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Affymetrix eBioscience
14-8863-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence. Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications

Specifications

TRA-1-60
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Unconjugated
Mouse
Human
Primary
4° C
0.5 mg/mL
Monoclonal
Liquid
RUO
IgM
Affinity chromatography
Antibody
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