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TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-8863-82

77.33 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15350080

  • £116.00 / 100µg
Estimated Shipment: 07-05-2024
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Description

Description

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Specifications

Specifications

TRA-1-60 (Podocalyxin)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Mouse
100 μg
Primary
4° C, do not freeze
Liquid
Flow Cytometry
TRA-1-60
Biotin
TRA160; tra60
Affinity chromatography
RUO
Human
Antibody
IgM
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