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TRA-1-60 (Podocalyxin), Biotin, clone: TRA-1-60, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-8863-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15350080

  • £87.34 / 100µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TRA-1-60 (podocalyxin)
TRA-1-60
Biotin
Conjugated
IgM
RUO
Primary
Human
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100μg
Store at 2-8°C. Do not freeze.
Monoclonal
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