TNF alpha, PE, clone: MP6-XT22, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7321-41

Product Code. 15557116

Description

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MP6-XT22 antibody is tested by intracellular flow cytometric analysis of stimulated splenocytes. This product has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: TNF alpha
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.2% BSA, 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P06804
• Gene Alias: DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: MP6-XT22
• Conjugate: PE
• Gene: Tnf
• Format: Conjugated
• Gene Symbols: Tnf
• Isotype: IgG1, kappa
• Quantity: 25 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 21926

For Research Use Only.