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TNF alpha, eFluor 450, clone: MP6-XT22, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7321-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15361830

  • £159.14 / 100µg


EU Stock 7
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Description

Description

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MP6-XT22 antibody has been tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06804
DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MP6-XT22
eFluor 450
Tnf
Conjugated
Tnf
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
21926
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