TNF alpha, Functional Grade, clone: MP6-XT22, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7321-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4^+ T cells. TNF-alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, neutralization, and immunohistochemistry.Applications Tested: The Functional Grade Purified MP6-XT22 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of TNF alpha bioactivity. The MP6-XT22 antibody at 1.25 μg/mL has been found to neutralize by 50% the biological effects of 1.0 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
|Store at 2-8°C.|
For Research Use Only.