TNF alpha, Biotin, eBioscience™
Brand: Affymetrix eBioscience 13-7326-85
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MP6-XT3 and MP6-XT22 antibodies react with mouse tumor necrosis factor-alpha (TNF-alpha ).Applications Reported: This cocktail antibody has been reported for use in detection of mouse TNF alpha by ELISA. Fluorochrome-conjugated formats of monoclonal antibody specific for mouse TNF alpha, MP6-XT22, (FITC, PE, APC) are effective for staining of intracellular TNF alpha. The Functional Grade purified format of monoclonal antibody specific for mouse TNF alpha, MP6-XT3 (cat. 16-7322), is effective for neutralizing biological activity of TNF alpha .Applications Tested: The biotinylated MP6-XT3 and MP6-XT22 antibody cocktail has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF alpha in combination with the purified 1F3F3D4 antibody (14-7325) for capture and recombinant mouse TNFa (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2.0μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Tumor Necrosis Factor alpha, TNFa TNF-a|