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TNF alpha, Biotin, eBioscience™

Antibody Cocktail

Brand:  Affymetrix eBioscience 13-7326-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15340060

  • £62.79 / 50µg


Estimated Shipment date
from Supplier 30-01-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The MP6-XT3 and MP6-XT22 antibodies react with mouse tumor necrosis factor-alpha (TNF-alpha ).Applications Reported: This cocktail antibody has been reported for use in detection of mouse TNF alpha by ELISA. Fluorochrome-conjugated formats of monoclonal antibody specific for mouse TNF alpha, MP6-XT22, (FITC, PE, APC) are effective for staining of intracellular TNF alpha. The Functional Grade purified format of monoclonal antibody specific for mouse TNF alpha, MP6-XT3 (cat. 16-7322), is effective for neutralizing biological activity of TNF alpha .Applications Tested: The biotinylated MP6-XT3 and MP6-XT22 antibody cocktail has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF alpha in combination with the purified 1F3F3D4 antibody (14-7325) for capture and recombinant mouse TNFa (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2.0μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
XT3/XT22
Biotin
Conjugated
Rat
50μg
Store at 2-8°C. Do not freeze.
Monoclonal
ELISA Detection
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Tumor Necrosis Factor alpha, TNFa TNF-a
IgG1
RUO
Primary
Murine
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