TNF alpha, Alexa Fluor 488, clone: MP6-XT22, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-7321-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, IHC, neutralization, and immunofluorescent intracellular staining with flow cytometric analysis. Fluorochrome conjugated MP6-XT22 antibody is recommended for use in intracellular staining.Applications Tested: This MP6-XT22 antibody has been tested by intracellular immunofluorescent staining and flow cytometric analysis of activated splenocytes cultured in the presence of monensin. This can be used at less than or equal to 0.12 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Tumor Necrosis Factor alpha|
For Research Use Only.