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TNF alpha, Alexa Fluor 488, clone: MP6-XT22, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-7321-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15312080

  • £152.25 / 100µg


EU Stock 5
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Description

Description

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane-bound form.Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, IHC, neutralization, and immunofluorescent intracellular staining with flow cytometric analysis. Fluorochrome conjugated MP6-XT22 antibody is recommended for use in intracellular staining.Applications Tested: This MP6-XT22 antibody has been tested by intracellular immunofluorescent staining and flow cytometric analysis of activated splenocytes cultured in the presence of monensin. This can be used at less than or equal to 0.12 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06804
DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MP6-XT22
Alexa Fluor 488
Tnf
Conjugated
Tnf
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
21926
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