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TNF alpha, Biotin, Polyclonal, eBioscience™

Rabbit Polyclonal Antibody

Brand:  Affymetrix eBioscience 13-7341-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15300070

  • £62.79 / 50µg


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26kDa form is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer.Applications Reported: This polyclonal antibody has been reported for use in ELISA and ELISPOT.Applications Tested: The biotinylated rabbit anti-mouse/rat TNF alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the purified anti-mouse/rat TNF alpha monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF alpha (14-8321) or rat TNF alpha (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Tumor Necrosis Factor alpha
IgG
RUO
Primary
Rat
ELISPOT Detection Antibody
Biotin
Affinity Purified
Rabbit
50μg
Store at 2-8°C. Do not freeze.
Polyclonal
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