TNF alpha, Biotin, Polyclonal, eBioscience™
Rabbit Polyclonal Antibody
Brand: Affymetrix eBioscience 13-7341-85
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26kDa form is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer.Applications Reported: This polyclonal antibody has been reported for use in ELISA and ELISPOT.Applications Tested: The biotinylated rabbit anti-mouse/rat TNF alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the purified anti-mouse/rat TNF alpha monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF alpha (14-8321) or rat TNF alpha (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze.|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
For Research Use Only.