TNF alpha Monoclonal Antibody (MAb11), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7349-81

Product Code. 15209329

Description

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody. Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MAb11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Specifications

• Antigen: TNF alpha
• Classification: Monoclonal
• Concentration: 0.2mg/mL; 5μL (0.25μg)/test
• Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Host Species: Mouse
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: MAb11
• Conjugate: PE
• Gene Alias: Tumor Necrosis Factor alpha
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.