TNF alpha, eFluor 450, clone: MAb11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-7349-42
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Tumor Necrosis Factor alpha|
For Research Use Only.