TNF alpha, APC, clone: MAb11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 17-7349-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Applications Reported: MAb11 has been reported for use in intracellular flow cytometric analysis.Applications Tested: This MAb11 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at test size: 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.2% BSA, 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.