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TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor™ 700, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£97.00 - £210.00
Specifications
Antigen | TNF alpha |
---|---|
Clone | MAb11 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15510377
|
Affymetrix eBioscience
56-7349-41 |
25 Tests |
£97.00
25 tests |
Estimated Shipment: 07-05-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
15520377
|
Affymetrix eBioscience
56-7349-42 |
100 Tests |
£210.00
100 tests |
Estimated Shipment: 18-04-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody. Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MAb11 antibody has been pre-titrated and tested byintracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.
TNF alpha Monoclonal antibody specifically detects TNF alpha in Human samples. It is validated for Flow Cytometry.Specifications
TNF alpha | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor | |
Tnf | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Tnf |
MAb11 | |
Flow Cytometry | |
Alexa Fluor 700 | |
Mouse | |
Human | |
P01375 | |
7124 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.