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TNF alpha, Alexa Fluor 700, clone: MAb11, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-7349-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15520377

  • £159.14 / 100 tests


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MAb11 antibody has been pre-titrated and tested byintracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TNF alpha
MAb11
Alexa Fluor 700
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Tumor Necrosis Factor alpha
IgG1
RUO
Primary
Human
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