Thermo Scientific™ TNF alpha ELISA Kit, Porcine

TNF alpha ELISA Kit, Porcine

Brand:  Thermo Scientific™ EP2TNFA2

Product Code. 15348204

Description

Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kit.

Features of the Porcine TNF alpha ELISA Kit:

Target: natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)

Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates

Measurement: absorbance at 450nm minus absorbance at 550nm

Assay Range: 31.3 to 2000 pg/mL

Sensitivity: < 5 pg/mL

Precision: dependent upon sample type and level (see instructions)

Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

Total Assay Time: 4 hours

Sample Size: 50μL

Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα rat TNFα bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.

This porcine TNF alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plates and lot-optimized reagents.

Specifications

• Assay Range: 31.3 to 2000pg/mL
• Description: TNF alpha ELISA Kit, 192 Assays, 3.1 to 2000pg/mL Test Range, 4 hr. Test Time, <0.5pg/mL Sensitivity, Cell Culture Supernatants, Plasma, Serum Sample, Swine/Porcine Species, Colorimetric Detection Method, TNFα Gene, Used for Protein Assays and Analysis
• Assay: 192
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Protein Family: Cytokines and receptors
• Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MI
• Sufficient For: 192 Assay
• For Use With (Application): Protein assays and analysis
• Kit Contents: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s) and stop solution
• Precision: Dependent upon sample type and level
• Regulatory Status: For research use only (RUO)
• Sample Volume: 50 μL
• Target: Natural and recombinant porcine TNFα±
• Total Assay Time: 4 hr.

• Assay Sensitivity: <5pg/mL
• Detectable Analytes: TNF-a (TNF)
• Detection Method: Colorimetric
• Product Type: TNF alpha ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Substrate Type: TMB
• Target Species: Porcine, Swine
• Incubation Time: 4 hr.
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 192 Assays
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Test Time: 4 hr.

For Research Use Only. Not for use in diagnostic procedures.