TNF alpha, clone: TN3-19.12, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7423-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, ELISPOT, IVCCA, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, cat. 16-7423, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (cat # 16-7325), is recommended.Applications Tested: The TN3-19.12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the biotinylated anti-mouse/rat TNF alpha polyclonal antibody (13-7341) for detection and recombinant mouse TNF alpha (14-8321) or rat TNF alpha as the standard. Product 14-8320 is a bioactive protein, but is not recommended for rat TNF alpha ELISA.] A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II.The Functional Grade purified TN3-19.12 antibody has been tested for neutralization of TNF alpha bioactivity. The fluorochrome-conjugated TN3-19.12 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis and can be used at 20 μL per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2|
|Cytokine NeutralizationþELISAþFlow Cytometry|
|Store at 2-8°C.|
For Research Use Only.