TLR4/MD2 Rat anti-Mouse, PE, Clone: MTS510, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-9924-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15219369

Quantity Price
1 £ 176.7 / 100µg
EU Stock 2
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Host Species Rat
Applications Flow Cytometry
Clone MTS510
Conjugate PE
Isotype IgG2a
Format Conjugated
Species Reactivity Mouse
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL
Gene Alias TLR4/ MD-2, TLR-4/ MD2, toll-like receptor MD2 complex
Monoclonal or Polyclonal Monoclonal
Regulatory Status RUO
Quantity 100μg
Primary or Secondary Primary
Antigen TLR4/MD2

The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (∽30 kDa) and TLR4 (∽100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex . Please contact eBioscience Technical Support for further information.