TLR4/MD2 Rat anti-Mouse, Clone: MTS510, Functional Grade, eBioscience™

Rat Monoclonal Antibody, Functional Grade

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 16-9924-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15288837

Quantity Price
1 £ 182.82 / 100µg
EU Stock 4
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Description and Specification

Specification

Applications Flow Cytometry
Applications Functional Assay
Isotype IgG2a
Monoclonal or Polyclonal Monoclonal
Conjugate Unlabeled
Format Purified
Antigen TLR4/MD2
Primary or Secondary Primary
Species Reactivity Mouse
Gene Alias TLR4/ MD-2, TLR-4/ MD2, toll-like receptor MD2
Regulatory Status RUO
Formulation aqueous buffer, no sodium azide
Quantity 100μg
Concentration 1mg/mL
Host Species Rat
Clone MTS510
Storage Requirements Store at 2-8°C.

The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (∽30 kDa) and TLR4 (∽100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex . Please contact eBioscience Technical Support for further information.